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Maintaining the health, expansion, and inventory of cell lines
Performing cryopreservation and thawing of mammalian cells
Purification of leukocytes from mammalian-derived samples (i.e., venous blood) via different density-gradient centrifugation methods and RBC lysis protocols
Staining of cells in tubes and plates for intracellular and extracellular antigens
Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents
Counting cells via hemocytometer and/or proprietary cell counters
Preparing buffers, media, and other reagents
Maintaining inventory of critical laboratory supplies (consumables and reagents) in the lab
Attend meetings, as needed
Requirements:
At least 2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines
Best practice experience doing antibody methods for antigen labeling (e.g., flow cytometry, western blots, ELISA, ELISPOT)
Experience working with primary cells derived from various mammalian species
Proficient with phase-contrast and brightfield microscopy
Excellent aseptic technique
Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies
Ability to calculate basic laboratory calculations
Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency
Ability to read and understand Standard Operating Procedures
Ability to interact with researchers for delivery of cells
Ability to maintain a clean work area
Knowledge of GLP/GDP guidelines
Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
Excellent written and oral communication skills
A minimum of a bachelor’s degree in Microbiology, Biology, Biochemistry, Immunology, or a related field