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The Nirschl and Kolb laboratories at the University of Wisconsin–Madison invite applications for a Scientist I or II to contribute to the discovery and development of novel PET imaging tracers targeting pathological protein aggregates in neurodegenerative diseases, including Parkinson’s disease, Alzheimer’s disease, frontotemporal dementia, and ALS. These efforts support multi-institutional collaborations focused on improving early diagnosis and therapeutic development.
Job Responsibility:
Lead discovery/optimization by setting up and executing custom tissue-based assays, including autoradiography and radioligand-binding assays, competition assays, and immunoassays or immunostaining, to evaluate compound binding affinity and specificity in human and animal biospecimens
Conduct radioligand displacement studies, immunohistochemical validation, and biochemical extraction of pathological proteins from brain tissue
Conduct surface plasmon resonance (SPR) experiments to quantify molecular interactions
Support lead compound optimization and translational imaging biomarker validation
Requirements:
Demonstrated expertise in tissue assay development, protein biochemistry, or molecular imaging
Ability to work in a collaborative, interdisciplinary environment
Track record of outstanding research accomplishments
Ability to work in a highly collegial and collaborative academic environment
Broad knowledge base in bioassay development
Histopathological characterization of brain tissue samples
Tissue homogenization, biochemical extraction and purification of pathological proteins from brain tissue samples
Radioligand binding assays using tissue slices, homogenates, and purified proteins
Autoradiography experiments for evaluating tracer binding
Surface Plasmon Resonance measurements for assessing molecular interactions