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NewLimit is seeking a Senior Research Associate to join our Immunology team. One of our initial therapeutic programs is developing an epigenetic reprogramming intervention to rejuvenate aged T lymphocytes for infectious and inflammatory disease applications. In this role, you will help develop culture systems and perform functional assays to evaluate the effectiveness of our therapeutic candidates and enable the development of new medicines.
Job Responsibility:
Collaborate with our Single Cell Technology, Epigenetic Editing, and Predictive Modeling teams to screen epigenetic reprogramming interventions for aged T cells
Support in vivo studies by assisting with tissue processing (e.g., lymphoid and non-lymphoid organs) for downstream cellular and molecular analyses
Contribute to the development and execution of immune-mediated disease models, with opportunities for hands-on in vivo work for candidates with relevant experience or interest
Isolate human and mouse T cell populations using FACS, MACS, and density gradient separation
Develop in vitro assays to assess the therapeutic activity of Treg and conventional T cells
Perform flow cytometry to characterize immune cell populations
Deliver epigenetic reprogramming perturbations to primary T cells using multiple transgenic manipulation methods (e.g., viral vector, lipofection)
Requirements:
BS in cell biology, immunology, or a related natural science and 2+ years of work experience in an industry setting
Proficiency with mammalian T cell culture
Proficiency performing flow cytometry and/or FACS independently, with experience in accompanying data analysis
Experience performing T cell functional assays (e.g., proliferation, cytokine, cytotoxicity)
Willingness to support in vivo studies through tissue processing, sample preparation, and downstream analyses
Exceptional organizational skills, as demonstrated by detailed record-keeping in lab notebooks and an ability to effectively carry out multiple ongoing projects or experiments
Nice to have:
Hands-on experience with in vivo animal studies, including dosing techniques, dissection, and organ collection
Experience with animal models of inflammation and autoimmune disease
Experience with primary immune cell isolation using MACS and/or density gradient separation (e.g., Ficoll, Percoll, Lymphoprep, etc.)
Experience with high-throughput screening of T cells or other primary immune cells
Experience with Treg assays and/or T cell differentiation protocols (e.g., inducible Tregs, Th1/Th2/Th17 polarization)
Experience using computational tools to automate routine data analysis (e.g., Python programming, Unix, R)
What we offer:
Health, dental, and vision insurance
401(k) with company match
Paid parental leave
Industry-leading paid time off (PTO) — 20 days/year for all employees + holidays