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The Moores Cancer Center (MCC) is one of just 57 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 28 departments, 6 schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health, Jacobs School of Engineering, School of Biological Sciences & the School of Physical Sciences), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University (SDSU), and the basic and translational research of the La Jolla Institute of Immunology (LJI). These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs. As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility. MCC's mission is to transform cancer care in our catchment area and beyond by driving exceptional scientific discoveries and innovations in prevention, detection, care, and training. MCC will make a global impact on improving health by reducing cancer burden through accelerated discovery and translation, compassionate and interdisciplinary care, education and community engagement, with the foundation of our core values - Excellence, Innovation and Risk-Taking, Collaboration, Diversity and Service.
Job Responsibility:
Perform tasks and assays to support research on tumor inflammation
Animal colony management, genotyping, breeding and weaning
In vivo animal experiments that assess the role of immune cells in cancer
Perform molecular biological, biochemical, and cell biological techniques including tumor cell culture, DNA and RNA isolation from cultured cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins
Analyze tissues and cells using flow cytometry, immunoblotting, gene expression and cell biology assays
Perform basic sectioning of tissues, immunohistochemistry, fluorescent microscopy, ELISA assays, and SDS PAGE analyses
Maintain and prepare accurate records of all experiments
Perform cell culture and propagation of tumor cell lines
Responsible for maintaining and inventory of frozen cell stocks and routine testing for mycoplasma contamination
Serve as the point of contact with UC San Diego Animal Care Program
Submission of protocols for review, organization of approved protocols along with mandated record keeping
Train new lab members on ACP rules and regulations and ensure that their training is up to date
Manage a mouse colony of approximately 100 cages, perform breeding, weaning and genotyping duties
Perform mouse tumor studies
Act as the Area Safety Coordinator for assigned laboratory space
Responsible for EH&S compliance of the laboratory and the required training of lab personnel
Prepare biosafety protocols for submission to EH&S committees for review
Preparing media and reagents, maintaining laboratory organization and laboratory supplies, purchasing laboratory supplies, and other critical functions as needed for the laboratory to run smoothly
Requirements:
Theoretical knowledge of cell biology and molecular biology and/or Bachelor's degree in Biology, Chemistry, or other related areas, and/or equivalent education and experience
Experience working with mice for procedures, including anesthesia and tumor cell administration
Experience preparing reagents and cells for animal studies, working with animal models of tumors, and performing tumor harvesting
Experience managing a mouse colony, weaning, and genotyping, maintaining accurate colony records, and conforming with all local and national IACUC regulations
Experience performing routine cell culture techniques, such as pipetting culture media onto growing human cells in culture plates and pipetting trypsin solution onto cell layers
Experience following basic research protocols and instructions closely
Experience with extracting DNA and RNA from specimens
Experience performing PCR and qPCR reactions
Experience with preparing chemical solutions, including performing gel electrophoresis by mixing gel solutions and pouring into gel molds
Ability to perform standard mathematic computations (calculate molarity) accurately
Knowledge of basic laboratory procedures and equipment, such as sterile techniques, preparation of buffers, centrifuges, balance pH meters and electrophoresis equipment
Experience in performing experimental protocols and in recommending modifications to procedures and protocols
Experience working with animal care protocols and laboratory safety coordination and compliance training
Ability to integrate information obtained from scientific journals for use in the laboratory and disseminate to other lab members
Ability to adhere to established UCSD guidelines for safety and environmental issues
Demonstrated ability in data management, analysis, interpretation, and description of scientific data in a publication format
Ability to accurately record experimental details and results
Organizational skills to order and maintain lab equipment and supplies
Experience and skill in using computers for basic word processing and data entry as well as PubMed and database searching and basic database analysis (e.g. BLAST)
Computer skills and experience working with Word, Excel, and other commonly used programs
Strong communication skills (both verbally and written) needed to interact professionally and effectively with a diverse population at all levels of organization in person, over the phone or in writing
Ability to use diplomacy, tact, and discretion in all interactions
Enthusiasm for learning and a genuine interest in scientific research
Willingness and ability to learn new skills, research methods, and scientific principles
Ability to work independently and cooperatively
Employment is subject to a criminal background check
Must be willing and able to work with small animals