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Research Assistant II- Endocrinology

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Boston Children's Hospital

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Location:
United States , Boston

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Contract Type:
Not provided

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Salary:

44657.60 - 69773.60 USD / Year

Job Description:

At Boston Children’s Hospital, the quality of our care – and our inclusive hospital working environment – lies in the diversity of our people. With patients from local communities and 160 countries around the world, we’re committed to reflecting the spectrum of their cultures, while opening doors of opportunity for our team. Here, different talents pursue common goals. Voices are heard and ideas are shared. Join us, and discover how your unique contributions can change lives. Yours included. Position Summary/ Department Summary: We currently have one position open to study a wide range of research topics including cell-based treatment of diabetes/obesity and intestinal stem cell differentiation. We are seeking highly motivated, curious, and enthusiastic individuals with excellent problem-solving skills. Responsibilities include quantitative PCR, Western blot analysis, immunofluorescence staining, and flow cytometry as well as cell culture. Basic laboratory skills are required and experience in a previous lab is a plus. Working occasional weekends may be warranted. Specific Area of Research: Intestinal stem cells, intestinal hormones, organoid culture

Job Responsibility:

  • Isolate intestinal crypts from patient biopsies when needed for new organoid lines
  • Perform general cell culture work, including human intestinal organoids, mouse intestinal organoids, and human intestinal cell lines
  • Perform general laboratory experiments (initially supervised): RNA isolation, PCR, quantitative PCR, western blots, immunofluorescence staining, and flow cytometry
  • Use web-based design tools to create novel CRISPR and over-expression lentiviral plasmids
  • Analyze and interpreting experimental results to help determine the need for repeat studies
  • Organize data into graphs for presentation which will be shown and discussed at weekly lab meetings
  • Maintain an organized and thorough lab notebook that records protocols, results, and analysis
  • Perform literature searches to help with the designing of new experiments
  • Contribute to the writing and editing of new manuscripts
  • Support general lab operations

Requirements:

  • A bachelor’s degree in STEM is required
  • One year of work experience is required
  • Previous experience in a basic science laboratory
  • Have a background using basic mathematical and scientific principles to solve problems
  • Have a background in using effective oral and written communication skills in a team setting

Nice to have:

  • master’s degree a plus but not required
  • experience in a previous lab is a plus
What we offer:
  • flexible schedules
  • affordable health, vision and dental insurance
  • child care and student loan subsidies
  • generous levels of time off
  • 403(b) Retirement Savings plan
  • Pension
  • Tuition and certain License and Certification Reimbursement
  • cell phone plan discounts
  • discounted rates on T-passes

Additional Information:

Job Posted:
March 26, 2026

Employment Type:
Fulltime
Work Type:
On-site work
Job Link Share:

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