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Molecular Biology Intern

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10x Genomics

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Location:
United States , Pleasanton

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Category:
Research and Development

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Contract Type:
Not provided

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Salary:

Not provided
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Job Description:

The intern will join the spatial proteomics R&D effort, focusing on building and optimizing high-throughput experimental workflows for screening custom proteins. They will work with display systems (e.g., yeast display) and surface-based assays to evaluate affinity, specificity, and performance of designed proteins across multiple formats.

Job Responsibility:

  • Develop, optimize, and execute high-throughput screening assays to evaluate custom proteins
  • Build and refine yeast surface display workflows (and/or related display platforms) for quantitative binder characterization using flow cytometry
  • Establish and compare surface-based screening formats
  • Implement and benchmark conjugation strategies that link proteins to DNA or other reporter molecules for multiplexed assays and barcoded readouts
  • Design and execute cloning strategies for custom protein libraries and individual variants
  • Construct and validate expression vectors for yeast and/or other systems, including QC of expression and display levels
  • Support expression and basic QC of selected binders
  • Plan and perform FACS-based and/or plate-based binder screening experiments, including appropriate controls, titrations, and replicates
  • Analyze high-dimensional assay data to rank candidates based on affinity, specificity, and other performance metrics
  • Where applicable, prepare samples and data for sequencing-based readouts and collaborate with others to interpret sequence–function relationships
  • Summarize findings in clear figures, tables, and internal reports, and present progress in regular team meetings
  • Work with computational design, protein production, and assay development teams to integrate experimental results into the design–build–test–learn cycle
  • Maintain detailed electronic lab notebook records, SOPs, and reagent documentation to ensure reproducibility and handoff
  • Contribute to a final internal report and presentation describing the developed workflows, experimental results, and recommendations for future development

Requirements:

  • Current PhD candidate in Biochemistry, Biophysics, Molecular Biology, Bioengineering, Chemical Biology, or a closely related field
  • Ideally within the final 1–2 years of their PhD program, with substantial hands-on experimental training in protein or molecular biology
  • Proven ability to independently design, execute, and troubleshoot experiments
  • Strong quantitative and analytical mindset, including comfort with large datasets from high-throughput screens
  • Clear written and verbal communication skills, with the ability to present complex results across disciplines
  • Highly organized, collaborative, and comfortable working in a fast-paced, interdisciplinary R&D environment
  • Strong molecular biology foundation: PCR, cloning (e.g., Gibson, Golden Gate), site-directed mutagenesis
  • Plasmid design, sequence verification, and basic construct troubleshooting
  • Experience with protein expression and characterization in at least one system (yeast, E. coli, or cell-free): Induction/optimization of expression, basic purification or lysate-based assays, SDS-PAGE/Western
  • Hands-on experience with high-throughput or quantitative assay formats, such as: Flow cytometry / FACS, plate-based binding assays (ELISA or similar), or cell-based functional assays
  • Comfort with data analysis and visualization
  • General awareness and experience with AI data analysis

Nice to have:

  • Experience with Python, R, or analysis tools such as GraphPad Prism is nice to have
  • Yeast surface display, phage display, ribosome/mRNA display, or other library-based screening technologies
  • Experience with conjugation and bioconjugation methods, for example: Cysteine-based conjugation (maleimide-thiol, halo tag, SNAP/CLIP, etc.)
  • Click chemistry (e.g., azide–alkyne, DBCO, strain-promoted cycloaddition)
  • DNA-barcoding of proteins, or other methods linking proteins to nucleic acids or reporters
  • Familiarity with translation-linked or puromycin-based conjugation methods, or related approaches that couple protein synthesis to covalent capture is highly desired
  • Background in protein engineering, antibody/nanobody development, or de novo mini-binder workflows
What we offer:
  • Generous time off
  • Competitive and comprehensive health benefits package
  • Competitive easy-to-use benefits that promote wellbeing and make your life easier
  • Family friendly policies like parental leave
  • Award-Winning Workplace

Additional Information:

Job Posted:
January 10, 2026

Work Type:
On-site work
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