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The successful candidate will contribute to an innovative project focused on developing a novel viral vector for gene and guide RNA delivery into cereal crops, including sorghum. Our lab recently identified a new plant RNA virus infecting cereals. This discovery provides a unique opportunity to explore the virus biology and leverage its potential as a delivery vector for virus-induced gene silencing (VIGS) and gene-editing applications in monocots, including major cereal species. The position offers excellent opportunities for both basic and translational research.
Job Responsibility
Design and perform advanced molecular cloning
Facilitate transient expression of modified viral RNAs in plants and protoplasts
Conduct advanced PCR-based assays, northern and western blot analysis
Constructing cDNA libraries for high-throughput sequencing and perform data analysis
Utilize confocal microscopy for research purposes
Collaborate effectively with team members and external partners and assist in mentoring graduate and/or undergraduate students as needed
Prepare scientific manuscripts reporting research methodology, results, and interpretations
Assist the PI in annual report preparation for the funding agency
Requirements
Ph.D. in Virology, Biotechnology, Plant Pathology, or a related field.
Strong background in molecular biology techniques including advanced molecular cloning
Experience in engineering viral vectors
Expertise in cDNA library preparation for high-throughput sequencing and data analysis
Experience in protoplast isolation and transfection
Excellent written and verbal communication skills, teamwork, and organizational abilities
Nice to have
Strong background in molecular biology techniques including advanced molecular cloning
Experience in engineering viral vectors
Expertise in cDNA library preparation for high-throughput sequencing and data analysis
Experience in protoplast isolation and transfection
Excellent written and verbal communication skills, teamwork, and organizational abilities